Accelerated genome engineering through multiplexing

Accelerated genome engineering through multiplexing.

Throughout the biological sciences, the past 15 years have seen a push toward the analysis and engineering of biological systems at the organism level. Given the complexity of even the simplest organisms, though, to elicit a phenotype of interest often requires genotypic manipulation of several loci. By traditional means, sequential editing of genomic targets requires a significant investment o...

Accelerated nanoscale magnetic resonance imaging through phase multiplexing

Accelerated Mechanical Engineering/MS

For Stu dents En ter ing the Oklahoma State System for Higher Ed u cat ion Sum mer 2016 through Spring 2017 GEN ERAL RE QUIRE MENTS Me chan i cal En gi neer ing (Stan dard) A675 Bach e lor of Sci ence in Mechan i cal En gi neer ing/ Mas ter of Sci ence (Me chan i cal Engr.) F675 To tal Credit Hours . . . . . . . . . . . . . . . . . . . . . 145-149•§ Min i mum Re ten tion/Grad u a tion Grade Poi...

Multiwavelength metasurfaces through spatial multiplexing

Metasurfaces are two-dimensional arrangements of optical scatterers rationally arranged to control optical wavefronts. Despite the significant advances made in wavefront engineering through metasurfaces, most of these devices are designed for and operate at a single wavelength. Here we show that spatial multiplexing schemes can be applied to increase the number of operation wavelengths. We use ...

Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection

Genome-scale engineering of living organisms requires precise and economical methods to efficiently modify many loci within chromosomes. One such example is the directed integration of chemically synthesized single-stranded deoxyribonucleic acid (oligonucleotides) into the chromosome of Escherichia coli during replication. Herein, we present a general co-selection strategy in multiplex genome e...